Neutralization titer biomarker for antibody-mediated prevention of HIV-1 acquisition - Nature Medicine

  • 📰 NatureMedicine
  • ⏱ Reading Time:
  • 24 sec. here
  • 2 min. at publisher
  • 📊 Quality Score:
  • News: 13%
  • Publisher: 53%

Ireland News News

Ireland Ireland Latest News,Ireland Ireland Headlines

By integrating the serum concentration of a broadly neutralizing antibody (bNAb) with its in vitro 80% inhibitory concentration, the PT80 biomarker may be used to guide target levels of bNAbs for effective prevention of HIV acquisition. HelpEndHIV

, was used to assess in vitro sensitivity to VRC01 of HIV-1 Env-pseudotyped viruses . Full details on pseudovirus stock preparation, the TZM-bl assay and calculation of serum neutralization titers are reported in the appendix of ref.Using RNA samples from AMP participants who had acquired HIV-1 infection, the genomic region of the acquired HIV-1 isolate coding for the complete Env glycoprotein was sequenced and ancassette plasmid was synthesized by Genewiz.

Additional assays were performed on the VRC01 drug product . Neutralization titers are expressed as the concentration of the VRC01 drug product at which RLU were reduced by either 50% or 80% relative to virus control wells after subtraction of background RLU in cell control wells. The VRC01 drug product was assayed against each HIV-1 Env-pseudotyped virus three times, at starting concentrations of 100 and 5 μg ml, using eight three-fold serial dilutions. HIV-1 PVO.

 

Thank you for your comment. Your comment will be published after being reviewed.
Please try again later.

HelpEndHIV If you can’t get diagnosed with hiv, you will never end hiv. Too many spreaders unfortunately.

We have summarized this news so that you can read it quickly. If you are interested in the news, you can read the full text here. Read more:

 /  🏆 451. in İE

Ireland Ireland Latest News, Ireland Ireland Headlines